Description
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TransfectionReagentfor AGSCells(GastricAdenocarcinoma,CRL1739)
- Abiodegradablepolymerbasedtrasfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- TransfectionkitincludesTransfectionEnhancerreagent
- Gentleenoughtobeusedforsinglecellanalysis
- DownloadinvitroAGStransfectionprotocol:[PDF]
- Download AGSCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforAGScellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast77%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsAGSTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
AGSCellLine:
Gastriccancerremainsdifficulttocureandisconsideredthethirdprimarycauseofdeathfromcancerworldwide.Becauseofthelackofearlysymptoms,itishardtodetectandisoftendiagnosedatanadvancedstage.Inhibitionofhistonedeacetylase(HDAC)wasfoundtosuppressthegrowthofgastricadenocarcinomacells,asperthe2015studypublishedintheInternationalJournalofOncology.siRNAtransfectionwasoneoftheresearchmethodsutilizedtoexploretheprogressofHDACregulationinthecancercells.TheAGSgastricadenocarcinomacelllineisextensivelyemployedinpreclinicalstudiesofstomachcancer.TheAGScelllinewasinitiallyestablishedin1979fromthestomachtissueofa54-year-oldCaucasianfemalewhosufferedfromgastricadenocarcinoma(stomachcancer).ThesecellsexhibitepithelialcellmorphologyandaretumOrigenic.Thepatientreceivednopriortherapybeforeremovalofthesecells.AGScellscouldbeusedforresearchrelatedtodifferentformsofstomachcancer.AltogenBiosystemsprovidesahighlysensitivepolymer-basedtransfectionreagentkitfortheAGSstomachcancercellline.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheAGScellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinAGScells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoAGScellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
- PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]
AGSTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1172)
- 1.5ml(Catalog#1173)
- 1.5mlCRISPR(Catalog#2105)
- 8.0ml(Catalog#1174)
