Description

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Transfection Reagent for HUH-7 Cells (Liver Cancer Cells)

• A biodegradable polymer based transfection reagent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
• Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
• High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
• Gentle enough to be used for single cell analysis
• Download in vitro Huh7 transfection protocol: [PDF]
• Download Huh7 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for Huh7 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 88% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems Huh-7 Transfection Protocol: [PDF]

Download MSDS: [PDF]

HUH-7 Cell Line:

According to the American Cancer Society, liver cancer has a much better prognosis for complete remission and survival, if diagnosed early. Furthermore, liver cancer caught early has a 50% 5-year survival rate, versus an only 3% survival rate if it is detected late and has spread to other organs. The HuH-7 hepatoma cell line consists of well-differentiated tumorigenic liver cells that are useful for carrying out transfection studies. The HuH-7 cell line was derived from a liver tumor of a 57-year-old Japanese male patient in 1982. HuH-7 is a type of immortal cell line, as hepatoma cells, they exhibit an epithelial cell morphology. In addition to being a suitable transfection host for multiple cellular and molecular biology applications, this cell line is extensively employed in liver cancer research. Common forms of liver cancer are usually caused by viral hepatitis (B or C) infection or cirrhosis. Altogen Biosystems offers polymer-based biodegradable transfection reagents for the HuH-7 hepatoma cell line.

Data:

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the Huh-7 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

Figure 2. Protein expression of GAPDH in HUH-7 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into HUH-7 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems is a life sciences company that manufactures over 100 cell type specific and pre-optimized transfection kits, electroporation buffers, and targeted in vivo delivery kits and reagents. Advanced formulation of reagents and optimized transfection protocols provide highly efficient intracellular delivery of biomolecules (proteins, DNA, mRNA, shRNA and siRNA, and small molecule compounds). Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #1775)
• 1.5 ml (Catalog #1776)
• 1.5 ml CRISPR (Catalog #2158)
• 8.0 ml (Catalog #1777)