Description
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TransfectionReagentforNCI-H292Cells(LungCarcinomaCells,CRL-1848)
- Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
- Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
- KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
- ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
- ReproducIBLetransfectionresults
- Workswellforstandardreversetransfectionandhigh-throughputapplications
- DownloadinvitroNCI-H292transfectionprotocol:[PDF]
- Download NCI-H292CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforNCI-H292 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast93%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsNCI-H292TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
NCI-H292CellLine:
NCI-H292cellsaresensitiveformanydifferentviruses,includingherpessimplexvirus,measlesvirus,mumpsaswellassomestrainsofinfluenzavirustypeA.ThismakesthemanexcellentsubstituteforMKcellswithregardtolaboratorysafety,paramyxovirusisolation,andeaseofuse,accordingtotheJournalofClinicalMicroBIOLOGy(Jun1993,Vol.31,pp.1504–1510)TheNCI-H292tumOrigeniccelllineisderivedfromalymphnodemetastasislungcellsofa32-year-oldfemalewithmucoepidermoidpulmonarycarcinomaandexhibitsepithelialmorphology.NCI-H292cellshavebeennegativelytestedforL-DOPAdecarboxylaseanddisplaythesamemucoepidermoidcharacteristicsasnormal(non-cancerous)cellsdo.NCI-H292cellssusceptibletotheHepatitisBvirus,makingthemvaluabletoolsforbiomedicalresearch.Thiscelllineisusedasinvitromodelinavarietyofmolecularandcellbiologyresearchrelatedtolungcancer.AltogenBiosystemsmanufactureshigh-efficiencytransfectionreagentkitsforthiscellline.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheNCI-H292cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinNCI-H292cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoNCI-H292cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
NCI-H292TransfectionKit
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6858)
- 1.5ml(Catalog#6859)
- 1.5mlCRISPR(Catalog#2179)
- 8.0ml(Catalog#7013)