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LIPID-basedInVivoTransfectionKitforRodents(Mouse,Rat)
- Cationiclipidliposome-basedsiRNAandplasmidDNAinvivodeliveryreagent
- Efficientdeliverytotheliver,pancreas,kidney,andcertaintumortypesviasystemicadmiNISTration
- Completeproductsupportinformationisavailableat:LIPIDInVivoTransfectionKit
Figure1.Systemicadministration(i.v.)ofLipid-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
Figure2.Intratumoraladministration(i.t.)ofLipid-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
POLYMER-basedInVivoTransfectionKitforRodents(Mouse,Rat)
- Biodegradablepolymer-basedinvivoreagent
- Efficientdeliverytothelung,liver,kidney,andspleenviasystemicadministration
- Completeproductsupportinformationisavailableat: POLYMERInVivoTransfectionKit
Figure3.Systemicadministration(i.v.)ofPolymer-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
Figure4.Intratumoraladministration(i.t.)ofPolymer-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
NANOPARTICLE-basedInVivoTransfectionKitforRodents(Mouse,Rat)
- Nanoparticle-basedreagent
- Efficientdeliverytotheheart,lung,liver,pancreas,kidney,andmultipletumortypesviasystemicadministration
- Completeproductsupportinformationisavailableat: NANOPARTICLEInVivoTransfectionKit
Figure5.Systemicadministration(i.v.)ofNanoparticle-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
Figure6.Intratumoraladministration(i.t.)ofNanoparticle-basedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
PEG-LiposomeInVivoTransfectionKitforRodents(Mouse,Rat)
- PEGylatedliposomebasedreagent
- Efficientdeliverytothespleen,kidney,pancreas,liver,andmultipletumortypesviasystemicadministration
- Completeproductsupportinformationisavailableat: PEG-LiposomeInVivoTransfectionKit
Figure7.Systemicadministration(i.v.)ofLiposome-PEGbasedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
Figure8.Intratumoraladministration(i.t.)ofLiposome-PEGbasedInVivoreagentconjugatedwithsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.TissueswerecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=6).
Tissue-targeted InVivoTransfectionReagentsforRodents(Mouse,Rat):
- LIVER-targetedinvivoreagent
- KIDNEY-targetedinvivoreagent
- PANCREAS-targetedinvivoreagent
AllAltogen®InVivo TransfectionKitssuppliedwithready-to-runtransfectionprotocolsthateliminatetheneedforextensivetransfectionoptimizationexperiments.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
- Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
- NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
- CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
- RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
- Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
- Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
- PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]
AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).