Description

ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).

Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).

Pancreas InVivoTransfectionReagent
siRNAand DNA pancreas invivodeliveryreagentforanimalresearch(mouse,rat)

ModesofadmiNISTration:

• Systemicintravenous(i.v.)injection
• Directintratumoral(i.t.)injection
• Intraperitoneal(i.p.)injection

Pancreas-targeted InVivoTransfectionReagent

• Biodegradablelipidliposome-basedreagent
• Liposomeconjugatedcomplexesarestableinserum(16h)
• Efficientdeliverytothepancreastissue(viasystemicadministration)andpancreastumors(viaintratumoraladministration)
• EfficientsiRNA,shRNA,microRNA,andplasmidDNAdelivery
• Minimaltoxicity
• Functionallyvalidatedinmice
• ApplicableforplasmidDNA/siRNAco-injection
• DownloadPancreas-targeted invivotransfectionprotocol:[PDF] [Word]
• DownloadPowerPointpresentationforPancreas-targetedinvivotransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

DATA

Figure1.Systemicadministration(i.v.)ofPancreasInVivoTransfectionReagentconjugatedwith80ugofchemicallymodifiedsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.Tissues(Pancreas,Brain,Muscle,Kidney,Tumor,Heart,Spleen,Lung)werecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=8).

Figure2.Intratumoraladministration(i.t.)ofPancreasInVivoTransfectionReagentconjugatedwith80ugofchemicallymodifiedsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.Tissues(Pancreas,Brain,Muscle,Kidney,Tumor,Heart,Spleen,Lung)werecollectedandRNAisolated48hoursafterfirstinjection.SampleswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.RibosomalRNAlevelswereusedtonormalizetheLaminA/Cdata.Dataaremeans±SD(n=8).

Figure3. Systemicadministration(i.v.)ofPancreasInVivoTransfectionReagentconjugatedwith80ugofchemicallymodifiedsiRNAtargetingLaminA/CmRNAornon-silencingcontrolsiRNAfollowingtherecommendedprotocol.Tissues(Liver,Brain,Muscle,Kidney,Tumor,Heart,Spleen,Lung)werecollectedandtotalproteinfractionisolated72hoursafterfirstinjection.SampleswereanalyzedbyWesternBlotAnalysisforLaminA/Cgeneexpressionlevels.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml–10injections(Catalog#5050)
• 1.5ml–30injections(Catalog#5051)
• 8.0ml–160injections(Catalog#5052)