Description
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Kidney In Vivo Transfection Reagent
siRNA and DNA kidney in vivo delivery reagent for animal research (mouse, rat)
Modes of administration:
- Systemic intravenous (i.v.) injection
- Direct intratumoral (i.t.) injection
- Intraperitoneal (i.p.) injection
Kidney-targeted In Vivo Transfection Reagent
- Nanoparticle-based liposomal reagent
- Nanoparticle/liposome conjugated complexes are stable in serum (16h)
- Efficient delivery to the kidney tissue (via systemic administration) and kidney tumors (via intratumoral administration)
- Efficient siRNA, shRNA, microRNA, and plasmid DNA delivery
- Minimal toxicity
- Functionally validated in mice
- Applicable for plasmid DNA/siRNA co-injection
- Download Kidney-targeted in vivo transfection protocol: [PDF] [Word]
- Download PowerPoint presentation for Kidney-targeted in vivo transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
DATA
Figure 1. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).
Figure 2. Intratumoral administration (i.t.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Animal model is NOD/SCID xenograft mouse bearing flank tumor of human origin (kidney cancer cell line). Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and RNA isolated 24 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=7).
Figure 3. Systemic administration (i.v.) of Kidney In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.
Kidney Transfection Reagent citation references:
- The Journal of Pathology. 2015 235(5):731–744. BDNF repairs podocyte damage by microRNA-mediated … Min Li et al [PDF]
- Nature Communications. 2016. 7:12076. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Badal et al [PDF]
- Journal of the American Society of Nephrology. 2016 Nov;27(11):3308-3319. Synaptopodin Limits TRPC6 Podocyte Surface Expression and Attenuates Proteinuria. Yu H et al [PDF]
- British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
- Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
- J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]
Kidney In Vivo Transfection Kit
Altogen Research Services:
Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (60+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).
Volume Options:
- 0.5 ml – 10 injections (Catalog #5070)
- 1.5 ml – 30 injections (Catalog #5071)
- 8.0 ml – 160 injections (Catalog #5072)
