Description
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TransfectionReagentforA204Cells(RhaBDomyosarcomaCells)
- Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- TransfectionkitincludesTransfectionEnhancerreagent
- Gentleenoughtobeusedforsinglecellanalysis
- DownloadinvitroA204transfectionprotocol:[PDF]
- DownloadA204CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforA204cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsA204TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
A204CellLine:
PertheAmericanCancerSociety,thereareapproximately350diagnosesofrhabdomyosarcoma(RMS),cancerofconnectivetissue,intheUSannually,morethanhalfoftheseareinchildrenyoungerthantenyears.RMSisarare,butextremelyaggressiveformofcancer,accountingforaround40%ofallrecordedsofttissuesarcomasinadults.FurThermore,itisconsideredthemostcommonsofttissuesarcomaamongchildren.RMShasnotbeenextensivelystudied;thus,selectinganappropriatecelllinewithdesiredcharacteristicsisparticularlyimportant.TheA204celllinehasproventobeasuitablehosttoperformRMSpreclinicalresearch.A204cellswerederivedfromthemuscletissueofaone-year-oldfemalewithrhabdomyosarcomain1973.Theseareepithelialcellswhichwerereportedtoformsmallmalignanttumorsinnudemiceconsistentwithembryonicrhabdomyosarcoma.A204cellshaveshownlimitedsensitivitytoNaturalKillercellcytotoxicity.Inanexaminationofthetranscriptionalandpost-transcriptionalregulationofthehumanlamininbeta-2chaininhumantumorcelllines,A204expressedthelamininbeta-2chainmRNAandsecretedlamininheterotrimerscontainingthebeta-2chain.A204hasbeenstudiedincultureforitscapacitytosynthesizecollagentypesandotherextracellularmatrixproteins.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheA204cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).
Figure2.ProteinexpressionofGAPDHinA204cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoA204cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
- Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
- NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
- CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
- RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
- Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
A204TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1703)
- 1.5ml(Catalog#1704)
- 1.5mlCRISPR(Catalog#2101)
- 8.0ml(Catalog#7019)
