Description
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TransfectionReagentfor AR42JCells(PancreaticCarcinomaCells,CRL-1492)
- Ananoparticle-basedliposomeformulation
- Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
- TransfectionEnhancerreagentprovidedwiththekit
- Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
- Generatephysiologicallyrelevantdatayoucantrust
- EffectiveforplasmidDNA/siRNAco-transfection
- Easy-to-usetransfectionprotocolwithreproducIBLeresults
- Lowcytotoxicity
- DownloadinvitroAR42Jtransfectionprotocol:[PDF]
- Download AR42JCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforAR42Jcellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast87%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedbyreal-timeRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsAR42JTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
AR42JCellLine:
PancreaticcancerremainsafataldiseaseaswellasasignificantpublichealthproblemduetoitsresistancetorADIotherapyandchemotherapy.FurThermore,ithasthelowest5-yearsurvivalrateamongalltypesofcancer,at3%,asstatedbyCancerResearchUK.PreclinicalresearchfornoveldrugsusingRNAinterferenceisawell-knownlaboratorytechniquethatcouldhelpresearchersfindnewtherapiesforpatientswiththisdeadlydisease.Anefficienttransfectionreagent,suchastheAR42JTransfectionKitforpancreaticcarcinomacells,offeredbyAltogenBiosystems,canimprovetheoutcomeofthisresearch.AR42Jisapancreaticcancercelllinethathasproventobeasuitablehostfortransfection.TheAR42JcelllinewasestablishedusingthepancreastissuefromaWistarrat(Rattusnorvegicus)thatsufferedfromapancreatictumor.ThistumOrigeniccelllineexhibitsanepithelialcellmorphologyandproducesamylaseandotherexocrineenzymes,whichisinducible.Ithasreceptorsforglucocorticoidandinsulin,whichfacilitateproduction.AR42Jcellsareknowntoreorganizetheirendoplasmicreticulum(ER)extensively.ThesecellsmakeforagoodtransfectionhostandcouldbeusefulinmanymolecularandcellularBIOLOGystudies.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheAR42JcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinAR42Jcells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoAR42JcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
- RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
- Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
- Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
AR42JTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1180)
- 1.5ml(Catalog#1181)
- 1.5mlCRISPR(Catalog#2106)
- 8.0ml(Catalog#1182)
