Description

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TransfectionReagentfor ASPC-1Cells(PancreaticBetaCells,CRL-1682)

• Ananoparticle-basedliposomeformulation
• Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
• TransfectionEnhancerreagentprovidedwiththekit
• Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
• Generatephysiologicallyrelevantdatayoucantrust
• EffectiveforplasmidDNA/siRNAco-transfection
• Easy-to-usetransfectionprotocolwithreproducIBLeresults
• Lowcytotoxicity
• DownloadinvitroASPC-1transfectionprotocol:[PDF]
• Download ASPC-1CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforASPC-1cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedby qRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsASPC-1TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

ASPC-1 CellLine:

Pancreaticcancerisoneofthemostchallengingcancerstodiagnose,astherenospecificandreliablescreeningtestsonthemarketthatcanhelpdetectthediseaseinearlystages.AccordingtotheAmericanCancerSociety,ifcaughtinitsearlystages,the5-yearsurvivalrateis27%,butifcancermetastasizesthenthisnumbergoesdownto11%.TheAsPC-1celllinecomprisingpancreaticbetacellsandisaninvaluabletoolforpancreaticcancerresearchaswellascommonlyemployedforstudyinghumaninfectionsrelatedtothepancreas.TheAsPC-1celllinewasreestablishedvianudemousexenograftsinitiatedwithcellsfromtheascitesofa62-year-oldCaucasianwomanwithmetastaticpancreaticadenocarcinoma.Thereestablishedepithelialcellsretainedtheiroriginalmorphology.Thistumorrepresentsanewmodelforhumanpancreaticcancer.AsPC-1cellsareusedinstudiesseekingpossibletreatmentsforpancreaticcancer,whichhasafive-yearsurvivalrateoflessthanonepercent.Forexamples,researchhasshownthattheplantalkaloidsanguinarineinducedapoptosisofAsPC-1cells.Inanotherstudy,immunoprecipitationandELISAusinghumanpancreaticcancercellsrevealedthepresenceofhighlevelsofDcR3proteininAsPC-1cells.ReducingendogenousDcR3levelsincreasesFasL-inducedapoptosisofhumanpancreaticcancercells.Anotherintriguingstudyindicatedthatdeuteriumoxide(D2O),alsocalledheavywater,inducedapoptosisofAsPC-1,andisconsideredapossiblefuturetreatmentagainstAsPC-1.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheAsPC-1cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).

Figure2.ProteinexpressionofCyclophilinBinAsPC-1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoAsPC-1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]

AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1709)
• 1.5ml(Catalog#1710)
• 1.5mlCRISPR(Catalog#2109)
• 8.0ml(Catalog#7023)