
Description
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TransfectionReagentfor ASPC-1Cells(PancreaticBetaCells,CRL-1682)
- Ananoparticle-basedliposomeformulation
- Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
- TransfectionEnhancerreagentprovidedwiththekit
- Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
- Generatephysiologicallyrelevantdatayoucantrust
- EffectiveforplasmidDNA/siRNAco-transfection
- Easy-to-usetransfectionprotocolwithreproducIBLeresults
- Lowcytotoxicity
- DownloadinvitroASPC-1transfectionprotocol:[PDF]
- Download ASPC-1CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforASPC-1cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.Transfectionefficiencywasdeterminedby qRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsASPC-1TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
ASPC-1 CellLine:
Pancreaticcancerisoneofthemostchallengingcancerstodiagnose,astherenospecificandreliablescreeningtestsonthemarketthatcanhelpdetectthediseaseinearlystages.AccordingtotheAmericanCancerSociety,ifcaughtinitsearlystages,the5-yearsurvivalrateis27%,butifcancermetastasizesthenthisnumbergoesdownto11%.TheAsPC-1celllinecomprisingpancreaticbetacellsandisaninvaluabletoolforpancreaticcancerresearchaswellascommonlyemployedforstudyinghumaninfectionsrelatedtothepancreas.TheAsPC-1celllinewasreestablishedvianudemousexenograftsinitiatedwithcellsfromtheascitesofa62-year-oldCaucasianwomanwithmetastaticpancreaticadenocarcinoma.Thereestablishedepithelialcellsretainedtheiroriginalmorphology.Thistumorrepresentsanewmodelforhumanpancreaticcancer.AsPC-1cellsareusedinstudiesseekingpossibletreatmentsforpancreaticcancer,whichhasafive-yearsurvivalrateoflessthanonepercent.Forexamples,researchhasshownthattheplantalkaloidsanguinarineinducedapoptosisofAsPC-1cells.Inanotherstudy,immunoprecipitationandELISAusinghumanpancreaticcancercellsrevealedthepresenceofhighlevelsofDcR3proteininAsPC-1cells.ReducingendogenousDcR3levelsincreasesFasL-inducedapoptosisofhumanpancreaticcancercells.Anotherintriguingstudyindicatedthatdeuteriumoxide(D2O),alsocalledheavywater,inducedapoptosisofAsPC-1,andisconsideredapossiblefuturetreatmentagainstAsPC-1.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheAsPC-1cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).
Figure2.ProteinexpressionofCyclophilinBinAsPC-1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoAsPC-1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
- Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
- NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
- CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
- RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
- Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
- Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
AsPC-1TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1709)
- 1.5ml(Catalog#1710)
- 1.5mlCRISPR(Catalog#2109)
- 8.0ml(Catalog#7023)