Description
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TransfectionReagentforAstrocytes(PrimaryAstrocyte Cells,Astroglia)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- Work inthepresenceofserum
- Aprovenreagentforestablishingstablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroAstrocytetransfectionprotocol:[PDF]
- Download AstrocyteCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforAstrocytecellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsAstrocyteTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
AstrocyteCells(PrimaryAstrocytesandAstroglia):
Thehumanbraincomprisestwomajorcelltypes:neuronsandglia.NeuronsareresponsIBLefortransmittingnervesignalswhilegliasafeguardneurons.Astrocytes,asortofglialcells,makeover50%ofallbraincells.IntheeventofanydistresstotheCentralNervousSystem(CNS),aprocesscalledgliosisistriggered.Gliosisinvolvestherapiddifferentiationofglialcellstoprovidemechanicalsupporttoneurons.However,excessivegliosisissymptomaticofvariousformsofbraincancer.PerthestudypublishedintheJanuary2016issueofTumorBIOLOGy,astrocytesplayanimportantroleincontrollingtheenvironmentaroundatumor.Astrocytesretardthedetectionofcancercellsbytheimmunesystemandhelpenhancetheirproliferation.Preclinicalresearchtargetingastrocytescanprovidevaluableinsightintomolecularpathwaysthatcancurbtumors.Transfectionisawell-regardedtechniquetoconductpreclinicalresearchinmanydifferentcells,includingastrocytes.
Astrocytesarestar-shapedglialcellsinthebrainandspinalcord.Astrocytesaresupportivecells,andtheirfunctionsincludebiochemicalsupportofendothelialcells,whichformtheblood-brainbarrier,providingnutrientstothenervetissue,extracellularK+levelregulation,neurotransmittersremoval,andhelpingrepairthebrainandspinalcordfollowingtraumaticinjuries.Theyarealsodependentonmyelinationandsynapsesformation.ManyprimaryastrocytesexpresstheintermediatefilamentglialfibrillaryacidicproteinorGFAP.Researchindicatesthatastrocytescommunicatewithneuronsthroughthereleaseofgliotransmittersviaacalciumionprocess.Therearethreetypesofastrocytesinthecentralnervoussystem:fibrous,protoplasmicandrADIal.Itisbelievedastrocytescellbodiesareseparateanddonotoverlap.
Astrocytes,alsoknownasastroglia,arecellsinlivingorganismsthatmakeuppartsofthenervoussystem,particularlyinthebrainandspinalcord.Theyworkwiththebody’snaturalresponsetotraumainthebrainandspinalcord,byrepairingandprovidingnutrientstoaffectedareas.Becauseofthis,astrocytesmaybeusefulinthestudyofspecificmedicaltreatmentsforpatientssufferingfromspinalcordinjuries.Thesecellsarecrucialtoresearchinthefieldofneuroscience.
Primarycellculturesareusedinbiologicalandgenetherapystudiesandserveasvaluablemodelsystemsthatmaymoreaccuratelyrepresentthebiologyofhealthycells.Manyculturedcelllines,aswellasthemajorityofprimarycellcultures,canbetransfectedwithexogenousnucleicacidswhenappropriatetransfectionapproachesareemployed.Sincethemajorityoftransfectionmethodscausessignificanttoxicityinprimarycellcultures,optimizingthisprocedure,specificallytheprotocolandreagentstobeutilized,isessentialfordevelopinganeffectivetransfectionstrategiesforagivencelltype.ALTOGEN®Kitsforprimarycellsandsensitivecelllineshavebeendesignedtohavesignificantlylowercytotoxicitythanotheralternatives.
Data:
Figure1.SiRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoactrocytecellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).
Figure2.ProteinexpressionofLaminA/CinAstrocytecells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoAstrocytecellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
SelectedAstrocyteTransfectionReagent productcitations:
- JournalofNeuroscience.2013 33(44).Foxo3aTranscriptionallyUpregulatesAQP4andInduces …Kapooretal [PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
AstrocyteTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1711)
- 1.5ml(Catalog#1712)
- 1.5mlCRISPR(Catalog#2110)
- 8.0ml(Catalog#1713)
