Description
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TransfectionReagentforbEnd.3 CellLine(BrainEndotheliomaCells,CRL-2299)
- Ananoparticle-basedliposomeformulation
- Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
- TransfectionEnhancerreagentprovidedwiththekit
- Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
- Generatephysiologicallyrelevantdatayoucantrust
- EffectiveforplasmidDNA/siRNAco-transfection
- Easy-to-usetransfectionprotocolwithreproducIBLeresults
- Lowcytotoxicity
- DownloadinvitrobEnd3transfectionprotocol:[PDF]
- Download bEnd-3CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforbEnd3 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
bEnd.3CellLine:
Strokeisalife-threateningdiseasethataffectsarteriesinthebrainandcanbecausedbytheblockageofthearteriesbyabloodclot,resultinginseizures.bEnd.3isabrainendothelialcelllinetakenfromamouse’s(Musmusculus)brain/cerebralcortex.Asendothelialcells,theymakeuptheinteriorsurfaceofbloodvesselsandlymphaticvessels,whichcirculateblood.Thesecellsformthestructurethatnotonlyhelpscarrynutrientstothebrainbutalsoremoveswaste.ThebEnd.3celllineisusedinavarietyofmolecularandcellBIOLOGyresearch.Studyofthiscellcouldbeusefulformedicalresearchrelatedtotheflowofblood,providingatestsubjectforwaystotreatclotsandcongestion,whichcancauseseizuresandotherserioushealtheffects.AltogenBiosystemsoffersanadvancedliposomeformulationbEnd-3transfectionreagentkitthatprovideshightransfectionefficiencywithbEnd-3cellsandisahelpfultoolforbiomedicalresearchapplicationsrelatedtotheflowofbloodandbloodflowobstruction.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRinthebEnd3cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinbEnd-3cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintobEnd-3cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
- JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
bEnd-3TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpreoptimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6145)
- 1.5ml(Catalog#6146)
- 1.5mlCRISPR(Catalog#2113)
- 8.0ml(Catalog#6147)
