Description
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TransfectionReagentforBeta-TC-6Cells(PancreasTumor,CRL-11506)
- Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
- Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
- KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
- Hightransfectionefficacyinthe presenceofserum
- ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
- ReproducIBLetransfectionresults
- Workswellforstandardreversetransfectionandhigh-throughputapplications
- DownloadinvitroBeta-TC6transfectionprotocol:[PDF]
- Download Beta-TC-6CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforBeta-TC6 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast91%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsBeta-TC6TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
Beta-TC-6CellLine:
Diabetesmellitusisadisorderthatoccurswhenthebodycannotmakegooduseoftheinsulinitproduces,orthepancreasfailstomakeenoughinsulin,whichresultsinhighbloodsugar.Insulinomaisarareneuroendocrinetumorthatformsinpancreaticisletcellsandismainlytreatedviasurgery.Therarityofinsulinomaposesachallengeinthedevelopmentofnoveltreatmentdrugsthatcanaddressit,astherearefewerparticipantsinclinicaltrials,whichmakescomprehensivepreclinicalstudiesespeciallycrucialinthequesttofindacureforit.Transfectionstudiescarriedoutonviablecancercelllinesarearobustandwidely-acceptedmethodforconductingpreclinicalresearchonalmostalltypesofcancer.TheBeta-TC-6celllinewasestablishedfrompancreatictumortissuefromatransgenicmouse(Musmusculus)sufferedfrominsulinoma.ThesecellsaretransgenicforSV40largeTantigenandexhibitepithelialcellmorphology(betacelltype).Theyhavebeenknowntoproduceglucagon,insulin,andsomatostatin,secretingmainlyinsulinspecificallyinresponsetothepresenceofglucose.Beta-TC-6cellsarebeneficialforbiomedicalresearchrelatedtodiabetes.
Data:
Figure1.GAPDHmRNAlevelswerequantifiedusingreal-timeRT-PCRintheBeta-TC6cellstransfectedwithsiRNAstargetingGAPDHornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDHmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinBeta-TC-6cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoBeta-TC-6cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Beta-TC6TransfectionKit
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
- PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6150)
- 1.5ml(Catalog#6151)
- 1.5mlCRISPR(Catalog#2114)
- 8.0ml(Catalog#6152)
