Description
ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).
Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).
TransfectionReagentforBHK-21Cells(KidneyCells,CCL-10)
- Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- TransfectionkitincludesTransfectionEnhancerreagent
- Gentleenoughtobeusedforsinglecellanalysis
- DownloadinvitroBHK-21transfectionprotocol:[PDF]
- Download BHK-21CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforBHK-21 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast86%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsBHK-21TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
BHK-21 CellLine:
Chronickidneyfailureisalossofkidneyfunctionoverthetimethatisconsideredasignificantpublichealthproblemworldwide.Developmentofnovelkidneytherapeuticsandinnovativetreatmentsoftendependsonpreclinicalstudiesthatcanhelptogaintheunderstandingoftheetiologyofthedisease.TheBHK-21celllinewasestablishedin1961from1-day-oldhamsterbyI.A.MacphersonandM.G.P.Stoker.Thiscelllineexhibitedfibroblastmorphologyandwastakenfromthekidneysofhealthygoldenhamster(Mesocricetusauratus).ThesecellsaresusceptIBLetohumanadenovirus25,poliovirus2,andreovirus3,amongothers.BHK-21cellsarebeneficialforstudyingmammaliankidneycellsBIOLOGy.
Adenovirusesbelongtoafamilyofvirusescharacterizedbythepresenceofnonenvelopeddouble-strandedDNAandtheabilitytoinfectawidevarietyofhumancells.Adenovirusespreferablytargetupperrespiratoryregionofthebody,andthesymptomsmanifestthemselvesintheformofhighlycontagiousdiseasessuchasconjunctivitis.Theyarealsoresponsibleformoresevereillnessessuchasmeningitis.Hamsterfibroblastswerefoundtobehighlypronetoinfectionadenoviruses,andthus,theBHK-21cellline.OvertimetheBHK-21hasproventobeareliabletransfectionhostduringpreclinicalstudiesinvolvingnotonlyadenovirusesbutotherpotentialpathogenssuchasreoviruses.AltogenBiosystemsprovidesbiodegradablepolymer-basedhighefficiencytransfectionreagentkitsthathaveexhibitedlowcytotoxicityandhighsiRNAuptakebyhostcells.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoBHK-21cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminA/CinBHK-21cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoBHK-21cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
BHK-21TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
- PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6175)
- 1.5ml(Catalog#6176)
- 1.5mlCRISPR(Catalog#2115)
- 8.0ml(Catalog#6177)
