Description
ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).
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TransfectionReagentfor BJCells(SkinFibroblastCells,CRL-2522)
- Ananoparticle-basedliposomeformulation
- Transfectionprotocolsprovidedfortransfectionofproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
- TransfectionEnhancerreagentprovidedwiththekit
- Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
- Generatephysiologicallyrelevantdatayoucantrust
- EffectiveforplasmidDNA/siRNAco-transfection
- Easy-to-usetransfectionprotocolwithreproducIBLeresults
- Lowcytotoxicity
- DownloadinvitroBJtransfectionprotocol:[PDF]
- Download BJCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforBJcellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency
Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsBJTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
BJ CellLine:
ThetwomainadvantagesofusingtheBJcelllineareitsmuchlongerLifespancomparedtootherfibroblastcellsaswellasupto72populationdoublingsbeforesenescence.TheBJfibroblasttelomerasenegativecelllinewasderivedfromtheforeskinofahealthynewbornmaleandisasuitablehostfortransfectionaswellasapowerfultoolfortheassessmentofcellularfunctionality.DermalfibroblastsfoundwithintheskinplayanessentialroleincellandmolecularBIOLOGyresearchregardinghealingofwounds,astheyareresponsibleforgeneratingconnectivetissuethatimpartselasticitytotheepidermis.AltogenBiosystemsprovidesnanoparticle-basedtransfectionreagentkitsfortheBJskinfibroblastcelllinethatyieldatleast73percenttransfectionefficiencyandarehighlyutilizedinmolecularandtelomerasebiologyresearch.ThereadytoorderAltogenBiosystemsBJtransfectionkitsofferastableperformanceandhavelowcytotoxicity.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRin BJcelllinetransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinBJcells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoBJcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
BJTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
- J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6210)
- 1.5ml(Catalog#6211)
- 1.5mlCRISPR(Catalog#2116)
- 8.0ml(Catalog#6212)
