Description
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TransfectionReagentforBxPC-3Cells(PancreaticCancerCells,CRL-1687)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- Work inthepresenceofserum
- Aprovenreagentforestablishingstablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroBxPC3 transfectionprotocol:[PDF]
- Download BxPC-3CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforBxPC3cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast92%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsBxPC3TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
BxPC-3CellLine:
Oneofthedifficultcancertypestodiagnose,pancreaticcanceralsohassomeofthelowest5-yearsurvivalrates.AccordingtoanarticlepublishedintheJournalofGeneralPhysiologyintheMarchof2009,CysticFibrosisTransmembraneConductanceReceptors(CFTR)provideapathwayforthetransportofbicarbonateionsacrosstheapicalmembraneofthepancreas.MalfunctioningofCFTRhasbeenlinkedtocancersthataffectpancreaticexocrinecells.Preclinicalstudiesofpancreaticcancer,oneofthedeadliestformsofcancer,canhelpresearchersidentifynoveltherapiesandprogressivetreatments,improvingthesurvivalratesforpatientsdiagnosedwiththisfataldisease.BxPC-3isapancreaticcancercelllinethatistumOrigenicandareliablehostforinvitrotransfectionstudies.TheBxPC-3celllinewasinitiallyestablishedusingpancreatictissuederivedfromasampleofa61-year-oldfemalepatientwithadenocarcinoma.ThesecellshavebeentestedpositiveforCFTRandfailtoexpressthecysticfibrosistransmembraneconductanceregulator(CFTR).BxPC-3cellspossessanepithelialcellularmorphology,aswellasmakeausefultransfectioninvitromodel.AltogenBiosystemsprovidesthelipid-basedBxPC-3transfectionreagentkitthathassuccessfullyaidedtheestablishmentofstableBxPC-3pancreaticcancercelllinesandisasuitablehostforstudyingmammalianembryonictissue.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheBxPC-3 cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinBxPC-3cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoBxPC-3cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
BxPC-3TransfectionKit
AltogenBiosystems:
AltogenBiosystemsprovidespreoptimizedtransfectionproductsforlifescienceresearchapplications.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,RNA,andanyothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6320)
- 1.5ml(Catalog#6321)
- 1.5mlCRISPR(Catalog#2119)
- 8.0ml(Catalog#6322)
