4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
Altogen Biosystems(授权代理)
主营:主营:高分子化学,分子和细胞生物学,转染试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Immunostain_staining_kit > Altogen/C2C12转染试剂(小鼠成肌细胞)/1.5 ml/6344
商品详细Altogen/C2C12转染试剂(小鼠成肌细胞)/1.5 ml/6344
Altogen/C2C12转染试剂(小鼠成肌细胞)/1.5 ml/6344
Altogen/C2C12转染试剂(小鼠成肌细胞)/1.5 ml/6344
商品编号: 6344
品牌: Altogen Biosystems
市场价: ¥10420.00
美元价: 6252.00
产地: 美国(厂家直采)
公司:
产品分类: 免疫组化染色试剂盒
公司分类: Immunostain_staining_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Description

Product Availability: In Stock (delivery within 2-3 business days, no extra charge for drop-shipping).

Ordering: To place an order via credit card or PO use Add to Cart button (creating an account is not required).


Transfection Reagent for C2C12 Cells (Mouse Myoblast Cells, CRL-1772)

  • Lipid-based transfection reagent
  • Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
  • Kit includes Transfection Enhancer reagent and recommended transfection protocol
  • High transfection efficacy in the presence of serum
  • Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
  • Reproducible transfection results
  • Works well for standard reverse transfection and high-throughput applications
  • Download in vitro C2C12 transfection protocol: [PDF]
  • Download C2C12 CRISPR/Cas9 transfection protocol: [PDF]
  • Download PowerPoint presentation for C2C12 cells transfection kit: [PPT]
  • Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 88% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems C2C12 Transfection Protocol: [PDF]

Download MSDS: [PDF]

C2C12 Cell Line:

Myocytes are long tubular cells that arise from myoblasts and make up muscle fibers in human beings. Subcloning is the targeted transfer of a specific DNA sequence from the parent vector to the target one. The C2C12 mouse myoblast cell line is a muscle tissue cell line that was derived from a subclone of a mouse (Mus musculus) cell line. C2C12 is a muscle tissue cell line, which exhibits myoblast morphology. This cell line acts as a good transfection model, but can rapidly differentiate into contractile myotubes, producing muscle-specific proteins. C2C12 has been tested negative for mousepox (ectromelia virus). These cells could be used for biomedical research and cell biology, especially of mammalian myoblast cells. Owing to its ability for rapid differentiation that can be easily modulated by treatment with proteins, the C2C12 cell line has proven to be a reliable transfection host for the study of muscle cells. Altogen Biosystems provides pre-optimized lipid-based transfection reagent kits, which have demonstrated high transfection efficiency verified by qRT-PCR, for the C2C12 mouse myoblast cell line.

Data:

C2C12 Cell Transfection Reagent

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the C2C12 cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPD mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=5).

C2C12-cells-transfection-protocol

Figure 2. Protein expression of GAPDH in C2C12 cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into C2C12 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):

  • Nature. 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
  • Hypertension. 2014 63(2):353-61. Tissue transglutaminase contributes to … Liu et al [PDF]
  • Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
  • Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
  • Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
  • PLoS Pathogens. 2012 8(8) Uridine composition of the poly-U/UC tract of HCV RNA … Schnell et al [PDF]
  • J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]
  • J Transl Med. 2010 15;8:133. Prevention of hyperglycemia-induced myocardial apoptosis … Zhang et al [PDF]
  • Mol Cell Biol. 2013 33(7). SCO2 induces p53-mediated apoptosis by Thr845 phosphorylation … Madan et al [PDF]
  • Hypertension. 2015 65(2):430-9. Neurokinin 3 receptor and phosphocholine transferase… Parchim et al [PDF]

Request a Quote for
C2C12 Transfection Reagent

Altogen Biosystems:
Altogen Biosystems transfection and electroporation products for life sciences and cancer research. Transfection reagents are developed for individual cancer cell line and transfection protocols are optimized for maximum delivery efficiency. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, mRNA, shRNA, siRNA, and other negatively charged biomolecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

  • 0.5 ml (Catalog #6343)
  • 1.5 ml (Catalog #6344)
  • 1.5 ml CRISPR (Catalog #2120)
  • 8.0 ml (Catalog #7025)
品牌介绍
Altogen Biosystems全国代理部分产品列表: 货号 产品 规格 价格(美元) 5030       1703 A204 Transfection Reagent (Rhabdomyosarcoma) 0.5ml 170 1704 A204 Transfection Reagent (Rhabdomyosarcoma) 1.5ml 348 窗体顶端 窗体底端   2101 A204 Transfection Reagent (Rhabdomyosarcoma) 1.5ml CRISPR 548 窗体顶端 窗体底端   7018 A204 Transfection Reagent (Rhabdomyosarcoma) 8.0ml 1044 窗体顶端 窗体底端   1167 A375 Cells Transfection Reagent (CRL-1619) 0.5ml 195 1168 A375 Cells Transfection Reagent (CRL-1620) 1.5ml 427 2102 A375 Cells Transfection Reagent (CRL-1621) 1.5ml CRISPR 627 1169 A375 Cells Transfection Reagent (CRL-1622) 8.0ml 1281 5030 Nanoparticle In Vivo Transfection Reagent 0.5 ml - 10 injections  445 5010 LIPID In Vivo Transfection Reagent 0.5 ml - 10 injections 405 5040 PEG-Liposome In Vivo Transfection Reagent 0.5 ml - 10 injections 465 5020 POLYMER In Vivo Transfection Reagent 0.5 ml - 10 injections 425 5050 Pancreas In Vivo Transfection Kit 0.5 ml - 10 injections  495 5060 Liver In Vivo Transfection Kit 0.5 ml - 10 injections  495 5070 Kidney In Vivo Transfection Kit 0.5 ml - 10 injections  495 1721 BMS2 Transfection Reagent (Bone Marrow Cells) 0.5 ml  231 6345 C6 Transfection Reagent (Glioma Cells, CCL-107) 0.5 ml  199 6803 DLD-1 Transfection Reagent (Colon Cancer, CCL-221) 0.5 ml  201 1775 HUH-7 Transfection Reagent (Liver Cancer Cells) 0.5 ml  179