Description
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TransfectionReagentforCaco-2Cells(ColonAdenocarcinomaCell,HTB-37)
- Abiodegradablepolymerbasedtrasfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,si/shRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- Gentleenoughtobeusedforsinglecellanalysis
- DownloadinvitroCaco-2 transfectionprotocol:[PDF]
- Download Caco-2CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforCaco-2cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
Reagentexhibitsatleast94%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsCaco2TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
Caco-2CellLine:
Theearliercoloncancerisdiagnosed,thebettertheoutlookforcompleterecoveryandsurvival.However,despitetherecentcancerbreakthroughs,itcanbeextremelychallengingtoincreaselifeexpectancyinpatientswithlate-stagecoloncancer.TheCaco-2celllineisestablishedfromapatientwithcolorectalcarcinomaandiswidelyknownforitsrapiddifferentiatingability.Withamodalchromosomenumberof96,manyofthesecellsarepolyploidy.TheCaco-2celllineissusceptIBLetohumanimmunodeficiencyvirus1andcouldbepotentiallyusefulforthestudyofcertaintypesofcoloncancerinhumans.Thiscelllinehasbeenknowntodoubleevery~62hours.Transfectionisapowerfulinvestigativeresearchtoolthatcanbeusedtostudymolecularandbiochemicalpathwaysincancercells.AltogenBiosystemsprovidesefficientbiodegradabletransfectionreagentkitsfortheCaco-2coloncancercellline.Apolymer-basedtransfectionCaco-2kitforcolonadenocarcinomacellsyieldsatleast94%transfectionefficiencyandisahelpfultoolforgeneexpressionstudiesaswellasforassessingcellularfunctionality.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeqPCRintheCaco-2cellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeqPCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinCaco-2cells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoCaco-2cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
SelectedCaco-2transfectionreagent citationreferences:
- PLoSOne.201410;9(7).SOX2expressionisregulatedbyBRAFandcontributestopoorpatientprognosisincolorectalcancer.Lundbergetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
- PLOSONE.2014.SOX2ExpressionIsRegulatedbyBRAFand …Lundbergetal[PDF]
Caco-2TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.OurBIOLOGyCROservices include:Xenograftservices(over60xenograftmodels),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6347)
- 1.5ml(Catalog#6348)
- 1.5mlCRISPR(Catalog#2122)
- 8.0ml(Catalog#6349)
