>
产品中心 >
Transfection >
Altogen/Capan-1 Transfection Reagent (Pancreatic Carcinoma)/8.0 ml/6414
Description
ProductAvailABIlity:InStock(deliverywithin2-3businessdays,noextrachargefordrop-shipping).
Ordering:ToplaceanorderviacreditcardorPOuseAddtoCartbutton(creatinganaccountisnotrequired).
TransfectionReagentforCapan-1Cells(PancreaticCarcinomaCells,HTB-79)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- Aprovenreagentforestablishingstablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroCapan1transfectionprotocol:[PDF]
- Download Capan-1CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforCapan-1cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast89%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
Capan-1CellLine:
Pancreaticcancerremainsoneofthedeadliesttumorswithapoorprognosisandlifeexpectancy.AccordingtotheNationalPancreaticCancerFoundation(NPCF),only5%ofpancreaticcancerpatientswillbestillalivefiveyearsafterthediagnosis.TheCapan-1celllinewasderivedfroma40-year-oldmalepatientofpancreaticcancer.ThesetumOrigeniccellsexhibitanepithelialcellmorphologyandmakesuitabletransfectionhosts.Capan-1cellshavebeenknowntoproducegastric-typemucin,andexpressCFTR(cysticfibrosistransmembraneconductanceregulator).TheCapan-1pancreaticcelllinehasproventobeareliablehostfortransfectionstudiesthataidinvestigativeresearchfornewdrugs.Inadditiontobeingasuitablehostforpancreaticcancerstudies,thiscelllineishelpfulinmolecularBIOLOGyaswellasindrugdiscoveryresearch.AltogenBiosystemsoffersadvancedcationiclipidformulationhigh-efficiencytransfectionreagentkitsfortheCapan-1pancreaticcancercellline.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheCapan-1 cellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinCapan-1cells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoCapan-1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
Capan-1TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):
- CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
- Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
- JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
- PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
- MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
- Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6412)
- 1.5ml(Catalog#6413)
- 1.5mlCRISPR(Catalog#2126)
- 8.0ml(Catalog#6414)
