Description
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TransfectionReagentforCFPEo(HTRpC)Cells(TracheaEpitheliumCells)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Work inthepresenceofserum
- Aprovenreagentforestablishingstablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroCFPEotransfectionprotocol:[PDF]
- Download CFPEoCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforCFPEocellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast73%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsCFPEoTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
CFPEo(HTRpC)CellLine:
Approximately14%ofallnewlydiagnosedcancersarelungcancersthatmainlyoccurinolderpeople,withanaveragepatientagebeingaround70yearsold,accordingtotheAmericanCancerSociety(ACS).TheCFPEocelllinewasestablishedusinghumantracheaepitheliumcells(alsoknownasHTEpCCellLine).HumanTracheaEpitheliumCells(HTEpC)areresponsIBLeforkeepingtheairwayscleanandprovidingproperlubricationforairpassage.HTEpCareusuallyimpactedintheeventofarespiratorydisorderandhencemakeforappropriateinvestigativeandtoxicologicalresearchtools.Thesetypesofcellsintheairwaysareresponsibleformaintaininghumidityaswellascleaningtherespiratorytract.Inadditiontobeinganimportanttargetfordrugs,toxins,andcarcinogens,CFPEocellsarebeneficialcellculturemodelsinbiomedicalresearchfortreatmentsofasthma,viralinfection,fibroticlungdisease,lungcancer,andcysticfibrosis.Consequently,CFPEoareeffectiveforinvestigatingthefunctionandpathologyoftherespiratorysystem.Theyarealsousedindruguptakeexperiments,toxicologicalandwoundhealingresearch,oncology,andcelldifferentiation.InvestigationsincludestudyingtheregulationofintracellularpH,thestimulanteffectofIL-1bonairwayepithelialcellgrowth,andhowhumanrhinovirusinfectionaffectsupregulation,orhastening,ofICAM-1expressiononepithelialcells.AltogenBiosystemsprovidestheCFPEo(HTRpC)transfectionreagentkitforhumantracheaepitheliumcellsthatyieldshightransfectionefficiency.
Data:
Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRintheCFPEOcellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2.ProteinexpressionofCyclophilinBinCFPEocells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoCFPEocellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
CFPEo(HTRpC)TransfectionKit
AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Companydevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits). Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1729)
- 1.5ml(Catalog#1730)
- 1.5mlCRISPR(Catalog#2127)
- 8.0ml(Catalog#1731)
