Description

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TransfectionReagentforChromaffinCells

• Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• Gentleenoughtobeusedforsinglecellanalysis
• DownloadinvitroChromaffintransfectionprotocol:[PDF]
• Download ChromaffinCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforChromaffincellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast75%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsChromaffinTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

ChromaffinCellLine:

ChromaffincellsarelocatedinthemedullaoftheadrenalglandsinmammalsandareresponsIBLeforseveralfunctions,suchasmonitoringcarbondioxideandoxygenlevelsinthebody,respondingtostressorregulatingthebloodpressure.Closelyrelatedtothenervoussystem,chromaffincellsareoneofthemostcommonlystudiedneuralcrestderivativesduetothevarietyofbioactivesubstancestheysecreteintothebloodstream,includingadrenaline,noradrenaline,andotherhormones.Adrenalglandsareendocrineglandsthatsecretehormonesintothebloodstream,toregulatethebody’sresponsetostressfulstimuli.Adrenalmedulla,thecentralregionoftheseglands,containsthecellsthatsynthesizeandsecretethesehormones.Neuroendocrinecellsreleasedifferenthormonesintothebloodstreaminresponsetonervesignals.Chromaffincellsaremainlytheactivationsiteofthebody’sflightorfightresponse.Thechromaffincelllinewasderivedfromhumanchromaffincellculturesandisalsoknownasmedullarychromaffincellssituatedinthesympatheticnervoussystem.“Chromaffin”isacombinationofthewords“chromium”and“affinity”;theyweregiventhisnamebecausetheyeasilypickupchromatinstain.InadditiontobeingusefulinthefieldsofcellularandmolecularBIOLOGy,chromaffincellsarevaluableforstemcellresearchandclinicaltransplantation.AltogenBiosystemsprovidesadvancednanoparticle-basedliposomeformulatedchromaffintransfectionreagentkitsforhumanchromaffincelllinesthatplayanessentialroleinbiomedicalresearch.Thereagentkitsarehighlysensitiveandhaveexhibitedlowcytotoxicity.

Data:

Figure1.CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRin ChromaffincellstransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedtransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=3).

Figure2.ProteinexpressionofCyclophilinBinChromaffincells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoChromaffincellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1733)
• 1.5ml(Catalog#1734)
• 1.5mlCRISPR(Catalog#2130)
• 8.0ml(Catalog#7031)