Description
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TransfectionReagentfor CLBPECCells(NeuroblastomaCells)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Aprovenreagentforestablishingstablecelllines
- DownloadinvitroCLBPECtransfectionprotocol:[PDF]
- Download CLBPECCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforCLBPECcellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast70%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsTransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
CLBPEC CellLine:
Neuroblastomaisarareformofcanceroustumorthatdevelopsfromneuroblasts,foundintheadrenalglandsandalmostalwaysoccursinchildren,accordingtoSt.Jude’sHospital.Neuroblastomacomprises50%ofallcancersthatarediagnosedininfants,beingthemostcommoncancerininfantsunder12monthsofage,withthemajorityofcasesdiagnosedbyage5,aspertheAmericanCancerSociety(ACS). Sinceapproximately70%ofneuroblastomapatientsarediagnosedatalatestage,innovativetreatmentoptionsandnewapproachesarevital.Neuroblastsarecellsfoundinfetusesthatultimatelydevelopintoneurons. TheCLBPECcelllinewasestablishedfromneuroblastomacellsofapatientandisapowerfultoolforperformingtransfectionstudiesinthequesttofindaviablecureforthedisease.CLBPECcellsarewidelyusedinthestudyofcancersthataffectyoungchildren.AltogenBiosystemsprovideslipid-basedhigh-efficiencytransfectionreagentkitsfortheCLBPECneuroblastomacelllinethatisanappropriatehostforstudyingpediatricbraintumors.
Data:
Figure1.GAPDmRNAlevelswerequantifiedusingreal-timeRT-PCRintheCLBPECcellstransfectedwithsiRNAstargetingGAPDornon-silencingsiRNA.Forty-eighthourspost-transfection,thecellswereharvestedandanalyzedbyreal-timeRT-PCRforGAPDmRNAexpressionlevels.Datawerenormalizedagainstthe18SrRNAsignal.Controlsampleswereeithermock-transfectedoruntreated.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=5).
Figure2.ProteinexpressionofGAPDHinCLBPECcells.DNAplasmidexpressingGAPDHorsiRNAtargetingGAPDHweretransfectedintoCLBPECcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
CLBPECTransfectionReagent
AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifesciencesandcancerresearch.Transfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykits(animaltransfection):1)Tissue-targetedreagents(deliveryofproteins,DNA,andRNAintoliver,pancreas,orkidneytissues),and2)Broadrangeinvivobiodistributionreagents(PEG-Liposomebasedreagent,Nanoparticle-basedinvivoreagent,Lipid-basedtransfectionkit,andPolymer-basedkit).Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#1735)
- 1.5ml(Catalog#1736)
- 1.5mlCRISPR(Catalog#2131)
- 8.0ml(Catalog#1737)
