Description

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TransfectionReagentforDetroit-551Cells(EmbryonicFibroblastCells,CCL-110)

• Abiodegradablepolymerbasedtransfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
• HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
• AchieverobustsiRNAuptakefordependablegenesilencing
• Effectivetransfectionunderconditionsofupto40%serum
• Gentleenoughtobeusedforsinglecellanalysis
• DownloadDetroit-551 transfectionprotocol:[PDF]
• DownloadDetroit-551_CRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforDetroit-551cellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast78%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsDetroit-551TransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

Detroit-551CellLine:

FibroblastsarethemostcommonconnectivetissuecellspresentinthebodythatareresponsIBLeformakingupthestructuralframework.EmbryonicstemcellsarecommonlyemployedforcellandmolecularBIOLOGyresearchandareimperativeforthepropergrowthofstemcells.TheDetroit-551celllinewasinitiallyestablishedin1965usingskintissuetakenfromtherelativelyhealthyfetusofaCaucasianfemale.Thesecellsexhibitafibroblastmorphologyandcelltypeandarefrequentlyusedasfeedercellsforhumanembryonicstemcellresearch.Also,thiscelllineisanadequatehostforstudiesrelatedtomammalianembryonictissue.DuetotheirabilitytohavetheirgrowthenhancedbythepresenceofTNF(tumornecrosisfactor)alpha,Detroit-551cellsarewidelyusedinbiomedicalresearchapplications.ThiscelllinecanbetransformedbySV40,andhaveaLifespanof~25passages.Detroit-551cellsserveascontrolsinthestudyofmutatedcellsastheyarenormal,buttheycanalsobequicklytransformedbyviruses.AltogenBiosystemsprovidespolymer-basedbiodegradabletransfectionreagentkitsfortheDetroit-551fibroblastcellline.Thetransfectionreagenthasexhibitedhighefficiencyandhighsensitivity.

Data:

Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoDetroit-551cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).

Figure2.ProteinexpressionofLaminA/CinDetroit-551cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoDetroit-551cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

AltogenBiosystems:
AltogenBiosystemsprovidespre-optimizedtransfectionkitsandelectroporationproductsforlifescienceresearch.Transfectionproductsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedtoenablehightransfection efficiencyofbiomolecules.AltogenBiosystemsdevelopedinvivodeliveryproductsforsmallanimalresearch,mouseandrattargetedtissuedelivery:livertargeted,pancreastargeted,kidneytargeted,PEG-Liposome-,Nanoparticle-,Lipid-,andPolymer-basedinvivotransfectionkits.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofbiomolecules.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#6801)
• 1.5ml(Catalog#6802)
• 1.5mlCRISPR(Catalog#2137)
• 8.0ml(Catalog#7035)