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Altogen/HCC1937 Transfection Reagent (Breast Carcinoma)/0.5 ml/6807
Description
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TransfectionReagentforHCC1937Cells(BreastCarcinomaCells,CRL-2336)
- Abiodegradablepolymerbasedtrasfectionagent–onceinsidethecell,thepolymerdegradesintosmallerlesstoxiccomponentsreducingcelltoxicity,facilitatingreleaseofthetransgene,andimprovingtransfectionefficiency
- OptimizedforintracellulardeliveryofplasmidDNA,siRNA,microRNA,andmRNA
- HightransfectionefficiencyofbothsiRNAandplasmidDNAwithoutcompromisingcellviability
- AchieverobustsiRNAuptakefordependablegenesilencing
- Effectivetransfectionunderconditionsofupto40%serum
- TransfectionkitincludesTransfectionEnhancerreagent
- DownloadinvitroHCC1937 transfectionprotocol:[PDF]
- Download HCC1937CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforHCC1937cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast84%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsHCC1937TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
HCC1937CellLine:
Breastcancerremainsthesecondprimarycauseofcancerdeathsinfemalesworldwide,withmorethan40,000fatalitiesannuallyintheUnitedStatesalone.However,breastcancerdeathratesinolderwomenhavebeendecreasingforthelastdecadeprimarilyduetosignificantadvancesinbreastcancermanagementanditsearlydiagnosis,asstatedbytheAmericanCancerSociety(ACS).Ductalcarcinomacomprisesabout80%ofallbreastcancerdiagnoses.Itbeginsinthemilkductsandspreadstosurroundingbreasttissue.Despitenoveltreatmentoptions,breastcancerremainsamongthetopcausesoffemaledeaths,globally.Researchcarriedoutusingtransfectioncanleadtorevelationsthatcouldhelpdevisecombinatorialtreatmentstocurebreastcancer.TheHCC1937celllineisderivedinitiallyfromthemammaryglandofa23-year-oldCaucasianfemalewithbreastcarcinomathatisassociatedwithgeneticpredisposition.HCC1937wasestablishedfromaprimaryductalTNMStageIIB,grade3carcinomain1996.Thistypeofbreastcancerisbelievedtohavegeneticlinksbecauseinmanycasesmultiplefamilymemberswillbeaffected.Thiscelllinecouldbeusefulforthestudyofdifferenttypesofbreastcancer.HCC1937’smodalchromosomenumberis100.Eachcelldoesn’tpossessafullyintactXorYchromosome.AltogenBiosystemsmanufacturespre-optimizedpolymer-basedbiodegradabletransfectionreagentkits.ThereagenthasexhibitedhightransfectionefficiencyfortheHCC1937breastcarcinomacellline.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoHCC1937 cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminA/CinHCC1937cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoHCC1937cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
HCC1937TransfectionReagent
AltogenBiosystems:
AltogenBiosystemsmanufacturerspreoptimizedtransfectionkitsforcancerresearch.Reagentsandtransfectionprotocolsareoptimizedforindividualcancercelllines.AltogenBiosystemsdevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliveryintoliver,pancreas,andkidneytissues),andinvivobiodistributionreagents(PEG-Liposome,Nanoparticle,Lipid,andPolymer-basedkits).Optimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,andRNAmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6807)
- 1.5ml(Catalog#6808)
- 1.5mlCRISPR(Catalog#2145)
- 8.0ml(Catalog#7042)
