Description

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Transfection Reagent for HCC1937 Cells (Breast Carcinoma Cells, CRL-2336)

• A biodegradable polymer based trasfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
• Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
• High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
• Achieve robust siRNA uptake for dependable gene silencing
• Effective transfection under conditions of up to 40% serum
• Transfection kit includes Transfection Enhancer reagent
• Download in vitro HCC1937 transfection protocol: [PDF]
• Download HCC1937 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for HCC1937 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 84% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems HCC1937 Transfection Protocol: [PDF]

Download MSDS: [PDF]

HCC1937 Cell Line:

Breast cancer remains the second primary cause of cancer deaths in females worldwide, with more than 40,000 fatalities annually in the United States alone. However, breast cancer death rates in older women have been decreasing for the last decade primarily due to significant advances in breast cancer management and its early diagnosis, as stated by the American Cancer Society (ACS). Ductal carcinoma comprises about 80% of all breast cancer diagnoses. It begins in the milk ducts and spreads to surrounding breast tissue. Despite novel treatment options, breast cancer remains among the top causes of female deaths, globally. Research carried out using transfection can lead to revelations that could help devise combinatorial treatments to cure breast cancer. The HCC1937 cell line is derived initially from the mammary gland of a 23-year-old Caucasian female with breast carcinoma that is associated with genetic predisposition. HCC1937 was established from a primary ductal TNM Stage IIB, grade 3 carcinoma in 1996. This type of breast cancer is believed to have genetic links because in many cases multiple family members will be affected. This cell line could be useful for the study of different types of breast cancer. HCC1937’s modal chromosome number is 100. Each cell doesn’t possess a fully intact X or Y chromosome. Altogen Biosystems manufactures pre-optimized polymer-based biodegradable transfection reagent kits. The reagent has exhibited high transfection efficiency for the HCC1937 breast carcinoma cell line.

Data:

Figure 1. siRNAs targeting Lamin A/C mRNA or non-silencing control siRNA were transfected into HCC1937 cells following the recommended protocol. At 48 hours post-transfection the cells were analyzed by qRT-PCR for Lamin A/C gene expression levels. 18S rRNA levels were used to normalize the Lamin A/C data. Values are normalized to untreated sample. Data are means ± SD (n=4).

Figure 2. Protein expression of Lamin A/C in HCC1937 cells. DNA plasmid expressing Lamin A/C or siRNA targeting Lamin A/C were transfected into HCC1937 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems manufacturers preoptimized transfection kits for cancer research. Reagents and transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery into liver, pancreas, and kidney tissues), and in vivo biodistribution reagents (PEG-Liposome, Nanoparticle, Lipid, and Polymer-based kits). Optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, and RNA molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #6807)
• 1.5 ml (Catalog #6808)
• 1.5 ml CRISPR (Catalog #2145)
• 8.0 ml (Catalog #7042)