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Altogen/HCT-116 Transfection Kit (Colon Carcinoma)/8.0 ml/7045

Description
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TransfectionReagentforHCT-116Cells(ColonCarcinomaCells,CCL-247)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- AprovenreagentforestablishingHCT-116stablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroHCT116transfectionprotocol:[PDF]
- Download HCT-116CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforHCT-116 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast90%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsHCT116TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
HCT-116CellLine:
Coloncancermostlyaffectsolderpeopleintheirlate60sandearly70s;however,theonlygroupthatisassociatedwithincreasedincidenceratesispeopleyoungerthanage50,aspertheColonCancerAlliance(CCA).TheHCT-116coloncancercelllineisessentialforresearchingbiochemicalpathwaysthataidtheproliferationofcoloncarcinoma.TheHCT-116celllinewasderivedfromepithelialtumOrigeniccolontissueofanadultmalewithcolorectalcarcinoma.Thesecellshaveamutationinthe13codonoftherasproto-oncogene,andthereforetheyareusefulforPCRassaysstudyingthiscodon.Theyarealsopositivefortransforminggrowthfactorbeta1andbeta2expression.Thiscelllineisusefulforbiomedicalresearchrelatedtocoloncancer.AltogenBiosystemsprovideshighlyefficientlipid-basedtransfectionreagentkitsfortheHCT-116coloncancercellline.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoHCT-116 cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminA/CinHCT-116cells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoHCT-116cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
HCT-116TransfectionKit
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6811)
- 1.5ml(Catalog#6812)
- 1.5mlCRISPR(Catalog#2148)
- 8.0ml(Catalog#7045)