Description

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TransfectionReagentforHMEC Cells(MammaryEpithelial,BreastCancerCells)

• Proprietarycationiclipidsformulation
• HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
• KitincludesTransfectionEnhancerreagent
• Work inthepresenceofserum
• Aprovenreagentforestablishingstablecelllines
• Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
• Download HMECtransfectionprotocol:[PDF]
• Download HMECCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforHMECcellstransfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast76%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsHMECTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

HMEC CellLine:

Breastcancerrepresentsthemostprevalentmalignancyamongwomen,withmorethan300,000newcasesdetectedeveryyear.Also,itresultsinover40,000deathsintheUnitedStatesannually,makingitaseriouspublichealthchallenge,accordingtotheAmericanCancerSociety.Breastcancerpreclinicalresearchisessentialfortheinvestigationoftumordevelopmentaswellasthetherapeuticpotentialofanewdrugcandidate.TheHMECbreastcancercelllineiswidelyconsideredtobeaneffectivetoolforcarryingoutcarcinogenscreening.TheHMEC(humanmammaryepithelialcancer)celllinewasderivedfromglandulartissueofafemalebreastcancerpatientandisasuitablehostforstudiesrelatedtobreastcancerbiomedicalresearch.HMECcelllinedisplayscuboidalmorphologywithacharacteristicpatternofswirls.ResearchapplicationsoftheHMECcelllineincludecarcinogenscreeningandthreedimensionalculturing.AltogenBiosystemsprovidespre-optimizedcationiclipid-basedtransfectionreagentkitsfortheHMECbreastcancercellline.

Data:

Figure1. siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoHMECcellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofLaminA/CinHMECcells.DNAplasmidexpressingLaminA/CorsiRNAtargetingLaminA/CweretransfectedintoHMECcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature.2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Am JPathology.2010177(4):1870-80.RoleofocularcomplementfactorHinamurinemodel…Lyzogubovetal[PDF]
• NatureBiotechnology.201129(4):341-5.DeliveryofsiRNAtothemousebrainby…Alvarez-Ervitietal [PDF]
• CancerResearch.201171(15):5144-53.InhibitionofmiR-193aexpressionby…Iliopoulosetal[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]
• Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
• BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• PLoSPathogens.20128(8)Uridinecompositionofthepoly-U/UCtractofHCVRNA…Schnelletal[PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• J TranslMed.201015;8:133.Preventionofhyperglycemia-inducedmyocardialapoptosis…Zhangetal[PDF]
• MolCellBiol.201333(7).SCO2inducesp53-mediatedapoptosisbyThr845phosphorylation…Madanetal[PDF]
• Hypertension.2015 65(2):430-9.Neurokinin3receptorandphosphocholinetransferase…Parchimetal [PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1769)
• 1.5ml(Catalog#1770)
• 1.5mlCRISPR(Catalog#2155)
• 8.0ml(Catalog#7049)