Description

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Transfection Reagent for HUVEC Cells (Human Umbilical Vein Endothelial Cells)

• Proprietary cationic lipids formulation
• High transfection efficiency of small RNA (siRNA, shRNA, miRNA), mRNA, pDNA
• Effective and robust intracellular delivery
• Kit includes Transfection Enhancer reagent
• Work in the presence of serum
• A proven reagent for establishing stable HUVEC cell lines
• Download HUVEC transfection protocol: [PDF]
• Download HUVEC CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for HUVEC cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 80% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems HUVEC Transfection Protocol: [PDF]

Download MSDS: [PDF]

HUVEC Cell Line:

Angiogenesis is the growth of new blood vessels that is naturally used for healing and reproduction. The body controls angiogenesis by secreting chemical signals that can stimulate the formation of new blood vessels as well as the repair of damaged ones. Excessive or insufficient blood vessel growth may cause balance disturbance in the body, resulting in various deadly and debilitating diseases, such as cancer, cardiovascular disease, diabetic ulcers, and many others. Angiogenesis inhibitors usually interfere with blood vessel formation.

The Human Umbilical Vein Endothelial Cell line (HUVEC) is a powerful tool in the study of various physiological processes such as angiogenesis. The HUVEC cell line was derived and established from human umbilical vein endothelial cells, which is also where it gets its name. This cell line very useful in the study of the interior of the umbilical cord. It is believed that HUVEC cells may play a role in angiogenesis, the formation of blood vessels from pre-existing vessels, which supply cancer growths with nutrients. Multiple research groups work on the development of cancer medicines by targeting angiogenesis. Endothelial cells play a key role in a diverse range of physiological processes and pathophysiological processes, including arterial disease and cancer development. HUVEC systems are commonly used for physiological and pharmacological investigations, such as macromolecule transport, fibrinolysis, and blood coagulation. HUVEC are responsive to cytokine stimulation in the expression of cell adhesion molecule. Altogen Biosystems provides an advanced formulation of a lipid-based HUVEC transfection reagent kit for umbilical vein endothelial cells.

Data:

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the HUVEC cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Figure 2. Protein expression of GAPDH in HUVEC cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into HUVEC cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Selected in vivo transfection product citations (ALTOGEN® IN VIVO Transfection Kits used in the following publications):

• Nature. 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF]
• Am J Pathology. 2010 177(4):1870-80. Role of ocular complement factor H in a murine model … Lyzogubov et al [PDF]
• Nature Biotechnology. 2011 29(4):341-5. Delivery of siRNA to the mouse brain by … Alvarez-Erviti et al [PDF]
• Cancer Research. 2011 71(15):5144-53. Inhibition of miR-193a expression by… Iliopoulos et al [PDF]
• RNA. 2010 16(11):2108-19. RNase L releases a small RNA from HCV RNA that refolds … Malathi et al [PDF]
• Diabetologia. 2012 55(7):2069-79. The p47phox- and NADPH oxidase organiser 1 … Youn et al [PDF]
• British Journal of Cancer. 2012 107(3):516-26. TIGAR induces p53-mediated cell-cycle … Madan et al [PDF]
• Hypertension. 2014 63(2):353-61. Tissue transglutaminase contributes to … Liu et al [PDF]
• Circulation Research. 2010 15;107(8). Kruppel-like factor-4 transcriptionally regulates … Cowan et al [PDF]
• Hypertension. 2012 59(1):158-66. Role of uncoupled endothelial nitric oxide synthase … Gao et al [PDF]
• Jounal of Biological Chemistry. 2012 287(4):2907. Chaperoning of mutant p53 protein … Gogna et al [PDF]
• PLoS Pathogens. 2012 8(8) Uridine composition of the poly-U/UC tract of HCV RNA … Schnell et al [PDF]
• J Proteome Res. 2012(11) Retinal proteome analysis in a mouse model of oxygen-induced … Kim et al [PDF]

Altogen Biosystems:
Altogen Biosystems manufacturers preoptimized transfection kits for cancer research. Reagents and transfection protocols are optimized for individual cancer cell lines. Altogen Biosystems developed two types of in vivo delivery kits for animal research: Tissue-targeted reagents (delivery into liver, pancreas, and kidney tissues), and in vivo biodistribution reagents (PEG-Liposome, Nanoparticle, Lipid, and Polymer-based kits). Optimized transfection protocols provide efficient intracellular delivery of proteins, DNA, and RNA molecules in vitro and in vivo. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #4106)
• 1.5 ml (Catalog #4107)
• 1.5 ml CRISPR (Catalog #2159)
• 8.0 ml (Catalog #4108)