Description

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Transfection Reagent for L6 Cells (Rat Myoblast Cells, CRL-1458)

• A biodegradable polymer based transfection agent – once inside the cell, the polymer degrades into smaller less toxic components reducing cell toxicity, facilitating release of the transgene, and improving transfection efficiency
• Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
• High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
• Transfection kit includes Transfection Enhancer reagent
• Gentle enough to be used for single cell analysis
• Download in vitro L6 transfection protocol: [PDF]
• Download L6 CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for L6 cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 84% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems L6 Transfection Protocol: [PDF]

Download MSDS: [PDF]

L6 Cell Line:

Rodent models play an essential role in preclinical research for testing drug candidates for their ability to improve muscle function. Rat cell lines have been extensively employed to explore mechanisms of muscle differentiation and function. The L6 rat myoblast cell line has proven to be an excellent research model in the investigation and identification of new drugs and suitable therapeutic targets. The L6 myoblast cell line was derived by Dr. Yaffe using rat (Rattus norvegicus) thigh muscle and resembles skeletal muscle tissue. Negatively tested for mousepox (ectromelia virus), the L6 cell line can fuse in a cell culture, creating multinucleated myotubes as well as striated fibers. Also, L6 cells have a propensity for high cellular variability. Altogen Biosystems provides biodegradable polymer-based pre-optimized transfection reagent kits for the L6 rat myoblast cell line. An indispensable tool for studying mammalian connective tissue, the L6 kit is equally efficient for single and multiple transfections.

Data:

Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the L6 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

Figure 2. Protein expression of Cyclophilin B in L6 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into L6 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems provides pre-optimized transfection kits and electroporation products for life science research. Transfection products are developed for individual cancer cell line and transfection protocols are optimized to enable high transfection  efficiency of biomolecules. Altogen Biosystems developed in vivo delivery products for small animal research, mouse and rat targeted tissue delivery: liver targeted, pancreas targeted, kidney targeted, PEG-Liposome-, Nanoparticle-, Lipid-, and Polymer-based in vivo transfection kits. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of biomolecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #6837)
• 1.5 ml (Catalog #6838)
• 1.5 ml CRISPR (Catalog #2165)
• 8.0 ml (Catalog #7055)