Description

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TransfectionReagentfor LNCaPCells(ProstateCarcinomaCells,CRL-1740)

• Ananoparticle-basedliposomeformulation
• Transfectionprotocolsprovidedfortransfectionofsmallproteins,DNA,mRNA,siRNA,shRNAandmicroRNA
• TransfectionEnhancerreagentprovidedwiththekit
• Producehigherlevelofrecombinantproteinexpressionwithminimaldisruptionofnormalcellfunction
• Generatephysiologicallyrelevantdatayoucantrust
• EffectiveforplasmidDNA/siRNAco-transfection
• Lowcytotoxicity
• DownloadinvitroLnCAPtransfectionprotocol:[PDF]
• Download LnCAPCRISPR/Cas9transfectionprotocol:[PDF]
• DownloadPowerPointpresentationforLnCAP transfectionkit:[PPT]
• DevelopedandmanufacturedbyAltogenBiosystems

TransfectionEfficiency:

Reagentexhibitsatleast85%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.

TransfectionProtocolandMSDS:

DownloadAltogenBiosystemsLNCaPTransfectionProtocol:[PDF]

DownloadMSDS:[PDF]

LNCaP CellLine:

Prostatecancerisconsideredthemostfrequentmalignancyandthethirdprimarycauseofcancer-relateddeathsamongmenintheUnitedStates,accordingtotheAmericanCancerSociety.Blackmaleshave74%higheroddsofdevelopingprostatecancercomparedtonon-Hispanicwhitemen.Withthe5-yearsurvivalrateofonly29%forpatientswithadvancedstagedisease,furtherpreclinicalstudiesforthedevelopmentofnewtreatmentregimensarenecessary.TheLNCaPhumanprostateadenocarcinomaepithelialcelllineisestablishedfromthesupraclavicularlymphnodemetastasisofaCaucasianmalein1977andcangrowinaggregatesorasasinglecell.TheLNCaPcellsgrowreADIlyinvitro,formclones,andareresistanttohumanfibroblastinterferon.Inadditiontobeingreceptivetohormones,thecelllinehasahighaffinityforandrogenreceptorsinthecytosolaswellasnuclearfractionsofcells.Thecelllineexpressesprostatespecificantigen,andestrogenreceptorscanbeseeninthecytosol.Thefrequencyofthetumordevelopmentandthetimeoftumorappearancearesignificantlydifferentformalesandfemales.Malemicedodeveloptumorssoonerandatthehigherfrequencythanfemales.Hormonalmanipulationsdemonstratethatincidenceoftumordevelopmentcorrelateswiththelevelsofserumandrogen.However,therateofthetumorgrowthisnotdependentonthegenderorhormonalstatus.TheLNCaPprostatecancercelllineiswidelyusedinclinicaloncologyresearch.Itisconsideredanappropriatehostforstudyingprostateandhormonefunctionsandisextensivelyusedindrugdiscoveryanddifferenttypesofbiomedicalapplications.AltogenBiosystemsoffersnanoparticle-basedtransfectionreagentkitsfortheLNCaPcellline.

Data:

Figure1.SiRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoLNCaPcellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=3).

Figure2.ProteinexpressionofLaminAinLNCAPcells.DNAplasmidexpressingLaminAorsiRNAtargetingLaminAweretransfectedintoLNCAPcellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.

SelectedcitationsforLNCAPtransfectionreagentusedinthefollowingpublications:

• PLoSOne.20116(1)IdentificationofdistinctivepatternsofUSP19-mediatedgrowthregulationinnormalandmalignantcells.LuY,BedardN,ChevalierS,WingS[PDF]
• RNA.201016(11):2108-19.RNaseLreleasesasmallRNAfromHCVRNAthatrefolds…Malathietal[PDF]

Selected invivo transfectionproductcitations(ALTOGEN® INVIVO TransfectionKits usedinthefollowingpublications):

• Nature2008454(7203):523-7.Innateimmunityinducedbycomposition-dependentRIG-I…Saitoetal[PDF]
• Hypertension.201463(2):353-61.Tissuetransglutaminasecontributesto…Liuetal[PDF]
• Circulation Research.201015;107(8).Kruppel-likefactor-4transcriptionallyregulates…Cowanetal[PDF]
• Hypertension.201259(1):158-66.Roleofuncoupledendothelialnitricoxidesynthase…Gaoetal[PDF]
• JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
• JProteomeRes.2012(11) Retinalproteomeanalysisinamousemodelofoxygen-induced…Kimetal[PDF]
• Gastroenterology.2011141(2) Differential typeIinterferon-mediatedautophagictrafficking…Desaietal[PDF]
• PLoSPathog.201410(10)ExosomesfromhepatitisCinfectedpatientstransmitHCV…Bukongetal[PDF]

AltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatmanufacturesover100celltypespecificandpre-optimizedtransfection kits, electroporationbuffers,andtargetedinvivo deliverykitsandreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovidehighlyefficientintracellulardeliveryofbiomolecules(proteins,DNA,mRNA,shRNA andsiRNA,andsmallmoleculecompounds).Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.

AltogenLabsResearchServices:

AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.CROservices include:Xenograftservices(60+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).

VolumeOptions:

• 0.5ml(Catalog#1789)
• 1.5ml(Catalog#1790)
• 1.5mlCRISPR(Catalog#2166)
• 8.0ml(Catalog#7056)