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Altogen/LoVo Transfection Reagent (Colon Adenocarcinoma)/8.0 ml/7057
Description
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TransfectionReagentforLoVoCells(ColonAdenocarcinomaCells,CCL-229)
- Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
- Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
- ReproducIBLetransfectionresults
- Workswellforstandardreversetransfectionandhigh-throughputapplications
- DownloadinvitroLoVotransfectionprotocol:[PDF]
- Download LoVoCRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforLoVocellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast94%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsLoVo TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
LoVo CellLine:
ColorectalcanceristhethirdmostcommonlydetectedcancerintheUnitedStates,reportedlyaccountingfornearly50,000fatalitiesannually,aspertheAmericanCancerSociety(ACS).TheLoVoepithelialcelllinewasisolatedin1971fromametastatictumorofa56-year-oldCaucasianmalewithcolorectaladenocarcinoma.LoVocellsexpressmultipleoncogenes,includingN-ras,H-ras,Myb,c-myc,K-ras,sisandfosgenes.Also,CC-3andCC-4tumor-specificnuclearmatrixproteinsareexpressed.However,thecellsarenegativeforexpressionofcolonantigen3andCSAp(CSAp-).TheLoVocelllinepresentsananeuploidkaryotypewithamodalnumberof49andadoublingtimeofapproximately37hours.AbroadrangeofLoVocellsshowsanextraBchromosomeaswellasalteredA2.TheLoVocelllineisasuitablehostforstudyingvarioustypesofcoloncancer.AltogenBiosystemsprovideshighlyefficienttransfectionreagentkitsfortheLoVocellline.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoLoVocellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminAinLoVocells.DNAplasmidexpressingLaminAorsiRNAtargetingLaminAweretransfectedintoLoVocellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
SelectedLoVotransfectionreagentcitations:
- Carcinogenesis.2014.AmechaNISTicstudyoftheproapoptoticeffectoftolfenamicacid …Jeongetal[PDF]
- Diabetologia.201255(7):2069-79.Thep47phox-andNADPHoxidaseorganiser1…Younetal [PDF]
LoVoTransfectionReagent
AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Companydevelopedtwotypesofinvivodeliverykitsforanimalresearch:Tissue-targetedreagents(deliverytoliver,pancreas,andkidneytissues),andbroadrangeinvivodeliveryreagents(PEG-Liposome,Nanoparticle-based,Lipid-based,andPolymer-basedkits). Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedmoleculesinvitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#6840)
- 1.5ml(Catalog#6841)
- 1.5mlCRISPR(Catalog#2167)
- 8.0ml(Catalog#7057)
