Description
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Transfection Reagent for U87 MG Cells (Glioblastoma Cells)
- Two component formulation enhances lipid mediated transfection efficiency
- Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
- Kit includes Transfection Enhancer reagent and recommended transfection protocol
- High transfection efficacy in the presence of serum
- Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
- Download in vitro U87 transfection protocol: [PDF]
- Download U87 CRISPR/Cas9 transfection protocol: [PDF]
- Download PowerPoint presentation for U87 cells transfection kit: [PPT]
- Developed and manufactured by Altogen Biosystems
Transfection Efficiency:
Reagent exhibits at least 81% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.
Transfection Protocol and MSDS:
Download Altogen Biosystems U87 Transfection Protocol: [PDF]
Download MSDS: [PDF]
U-87 MG Cell Line:
Glioblastoma is a highly malignant tumor that originates from astrocytes and can be extremely difficult to eradicate due to it comprising many different cell types. Some of these cells react to treatment well, and others can be utterly unresponsive to drugs, as stated by the American Brain Tumor Association (ABTA). Human cell line models may lead to breakthroughs in brain cancer research and innovative treatment approaches that are essential for glioblastoma patients. U-87 MG is a human glioblastoma cell line derived from a stage three 44-year-old Caucasian female. It has epithelial morphology. Grade IV glioma, also called glioblastoma multiforme (GBM), is the most common primary malignant brain tumor with about 16,000 new diagnoses each year in the United States. GBMs have a one-year survival rate of only 29.6% making it one of the deadliest types of cancer. U-87 is a hypodiploid human cell line with the modal chromosome number of 44 that occurs in 48% of cells. The rate of higher ploidy is nearly 5.9%. Twelve markers are similar in all cells. There is only one copy of normal X, with N1, N6 and N9 being absent. U-87 MG cells are used as in vitro models of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The U-87 glioblastoma cell line is widely used in transfection studies and
