Description
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TransfectionReagentforU87MGCells(GlioblastomaCells)
- Twocomponentformulationenhanceslipidmediatedtransfectionefficiency
- Optimizedeasy-to-usetransfectionprotocolprovidedfortransfectionofsiRNA,DNA,mRNA,andmicroRNA
- KitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
- Hightransfectionefficacyinthe presenceofserum
- ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
- DownloadinvitroU87 transfectionprotocol:[PDF]
- Download U87CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationforU87 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast81%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsU87TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
U-87MGCellLine:
GlioblastomaisahighlymalignanttumorthatoriginatesfromastrocytesandcanbeextremelydifficulttoerADIcateduetoitcomprisingmanydifferentcelltypes.Someofthesecellsreacttotreatmentwell,andotherscanbeutterlyunresponsivetodrugs,asstatedbytheAmericanBrainTumorAssociation(ABTA).Humancelllinemodelsmayleadtobreakthroughsinbraincancerresearchandinnovativetreatmentapproachesthatareessentialforglioblastomapatients.U-87MGisahumanglioblastomacelllinederivedfromastagethree44-year-oldCaucasianfemale.Ithasepithelialmorphology.GradeIVglioma,alsocalledglioblastomamultiforme(GBM),isthemostcommonprimarymalignantbraintumorwithabout16,000newdiagnoseseachyearintheUnitedStates.GBMshaveaone-yearsurvivalrateofonly29.6%makingitoneofthedeadliesttypesofcancer.U-87isahypodiploidhumancelllinewiththemodalchromosomenumberof44thatoccursin48%ofcells.Therateofhigherploidyisnearly5.9%.TwelveMarkersaresimilarinallcells.ThereisonlyonecopyofnormalX,withN1,N6andN9beingabsent.U-87MGcellsareusedasinvitromodelsofhumanglioblastomacellstoinvestigatethecytotoxiceffectofchemotherapeuticdrugstowardscancercells.TheU-87glioblastomacelllineiswidelyusedintransfectionstudiesand