Description

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Transfection Reagent for VERO Cells (African Green Monkey Kidney Cells)

• Two component formulation enhances lipid mediated transfection efficiency
• Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
• Kit includes Transfection Enhancer reagent and recommended transfection protocol
• High transfection efficacy in the presence of serum
• Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
• Reproducible transfection results
• Works well for standard reverse transfection and high-throughput applications
• Download in vitro VERO transfection protocol: [PDF]
• Download VERO CRISPR/Cas9 transfection protocol: [PDF]
• Download PowerPoint presentation for VERO cells transfection kit: [PPT]
• Developed and manufactured by Altogen Biosystems

Transfection Efficiency:

Reagent exhibits at least 90% transfection efficiency of siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Transfection Protocol and MSDS:

Download Altogen Biosystems VEROTransfection Protocol: [PDF]

Download MSDS: [PDF]

VERO Cell Line:

Continuous mammalian cell lines serve as potent tools for life sciences, aiding in the production of various drugs. The Vero cell line is susceptible to multiple viruses and has widely contributed to the creation of vaccines for human use. The Vero epithelial cell line is taken from kidney cells of an African green monkey (Cercopithecus aethiops). Yasumura and Kawakita isolated these cells in 1962 and named the cell line as an abbreviation of “Verda reno” meaning “green kidney” in Esperanto. Vero cells are aneuploidy, continuous, and can be replicated through numerous cycles of division without becoming senescent. Aneuploidy means having an abnormal number of chromosomes. Unique from mammalian cells, the Vero cell line is interferon-deficient since it does not produce type 1 interferons after being infected by viruses. Vero cells are used for multiple purposes, including screening for the toxins such as Escherichia coli. Also, Vero can serve as a host cell line for growing viruses or eukaryotic parasites (Trypanosomatids.) The Vero cell line is a mammalian cell line commonly used for research in the fields of virology, bacteriology, and toxicology. Also, the Vero cell line is an excellent host for studying a range of areas within the field of biomedical therapeutics. Altogen Biosystems provides highly efficient pre-optimized transfection kits for these cells.

Data:

Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the VERO cells transfected with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated. Values are normalized to untreated sample. Data are means ± SD (n=3).

Figure 2. Protein expression of GAPDH in VERO cells. DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into VERO cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

Altogen Biosystems:
Altogen Biosystems is a life sciences company that offers cell type-specific and pre-optimized transfection products, electroporation kits, and in vivo delivery reagents. Advanced formulation of reagents and optimized transfection protocols provide efficient intracellular delivery of protein, DNA, mRNA, shRNA and siRNA molecules. Read more about transfection technology at Altogen’s Transfection Resource.

Altogen Labs Research Services:

Altogen Labs provides GLP-compliant contract research studies for pre-clinical research, IND applications, and drug development. Biology CRO services include: Xenograft models (30+), development of stable cell lines, ELISA assay development, cell-based and tissue targeted RNAi studies, safety pharm/tox assays, and other studies (visit AltogenLabs.com).

Volume Options:

• 0.5 ml (Catalog #3281)
• 1.5 ml (Catalog #3282)
• 1.5 ml CRISPR (Catalog #2201)
• 8.0 ml (Catalog #7092)