Description
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TransfectionReagentforWeri-Rb-1Cells(RetinoblastomaCells)
- Proprietarycationiclipidsformulation
- HightransfectionefficiencyofsmallRNA(siRNA,shRNA,miRNA),mRNA,pDNA
- Effectiveandrobustintracellulardelivery
- KitincludesTransfectionEnhancerreagent
- Producesconsistentresults,lot-to-lot,plate-to-plate,andwell-to-well
- Work inthepresenceofserum
- Aprovenreagentforestablishingstablecelllines
- Optimizedtransfectionprotocolsareadaptedforusewithbothstandard&reversetransfectionmethods
- DownloadinvitroWeriRb1transfectionprotocol:[PDF]
- Download Weri-Rb-1CRISPR/Cas9transfectionprotocol:[PDF]
- DownloadPowerPointpresentationfor Weri-Rb-1 cellstransfectionkit:[PPT]
- DevelopedandmanufacturedbyAltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast75%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCR.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsWeriRb1TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
Weri-Rb-1CellLine:
TheWERI-Rb-1celllinewasisolatedfromretinoblastomacellsofa1-year-oldCaucasiangirlbyR.M.McFallandT.W.Seryin1974.WERI-Rb-1isconsidereddiploidwithamodalchromosomenumberof47thatoccursat38%.Therateofpolyploidyisapproximately9%.AllcellscontainfifteentosixteenMarkerchromosomes,butnormalchromosomes3,10,13and16aremissing.Also,twocopiesoftheXchromosomearepresent,butYchromosomescouldnotbedetectedinQMstainedpreparations.WERI-Rb-1cellscansurviveculturinginDifcoBacto-Agar.WERI-Rb-1cellsareroundcellsthatgrowingrape-likeclustersinsUSPensionanddonotformcolonies.Scanningelectronmicroscopyshowsvariationsinthenumberofsurfaceblebs,microvilli,andlamellipodia.WERI-Rb1cellscouldnotbeclonedinsoftagar,andthepopulationdoublingtimeforthesecellsisroughly96hours.TheWERI-Rb-1celllineishighlyutilizedincelldifferentiationresearchandstudiesrelatedtoanimaltumortherapeutics.TheWERI-RB-1humanretinoblastomacelllineisparticularlyusefulinthestudyofbiochemicalpathwaysleADIngtocelldifferentiation.AltogenBiosystemsoffersrobustlipid-basedtransfectionkitsfortheWERI-RB-1celllinethatworksequallywellforbothstandardreversetransfectionaswellashigh-throughputapplications.
Data:
Figure1.siRNAstargetingLaminA/CmRNAornon-silencingcontrolsiRNAweretransfectedintoWeri-Rb1cellsfollowingtherecommendedprotocol.At48hourspost-transfectionthecellswereanalyzedbyqRT-PCRforLaminA/Cgeneexpressionlevels.18SrRNAlevelswereusedtonormalizetheLaminA/Cdata.Valuesarenormalizedtountreatedsample.Dataaremeans±SD(n=4).
Figure2.ProteinexpressionofLaminAinWeri-Rb-1cells.DNAplasmidexpressingLaminAorsiRNAtargetingLaminAweretransfectedintoWeri-Rb-1cellsfollowingAltogenBiosystemstransfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
SelectedWeri-Rb1transfectionreagentcitations:
- BMCCancer.2012 12(69).Regulationofp14ARFexpressionbymiR-24…Kwong-HimTo etal[PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- JounalofBIOLOGicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
Weri-Rb-1TransfectionReagent
AltogenBiosystems:
AltogenBiosystemstransfectionandelectroporationproductsforlifesciencesandcancerresearch.Transfectionreagentsaredevelopedforindividualcancercelllineandtransfectionprotocolsareoptimizedformaximumdeliveryefficiency.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofproteins,DNA,mRNA,shRNA,siRNA,andothernegativelychargedbiomoleculesin vitroandinvivo.Readmore abouttransfectiontechnologyatAltogen’sTransfectionResource.
AltogenResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.5ml(Catalog#3247)
- 1.5ml(Catalog#3248)
- 1.5mlCRISPR(Catalog#2202)
- 8.0ml(Catalog#7093)
