Description
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TransfectionReagentforPrimaryCellsandDifficult-to-TransfectCellLines
- Twocomponentformulationenhancesliposomemediatedtransfectionefficiency
- Transfectionprotocolprovidedfortransfectionofsi/miRNA,plasmidDNA,mRNA,andsmallproteins
- AltoFectTransfectionKitincludesTransfectionEnhancerreagentandrecommendedtransfectionprotocol
- ExpandyourRNAiapplicationwithareagentoptimizedfordeliveryofbothsiRNAandplasmid
- Download AltoFect™transfectionprotocol:[PDF]
- Developedandmanufacturedby AltogenBiosystems
TransfectionEfficiency:
Reagentexhibitsatleast85%transfectionefficiencyofsiRNAdelivery.TransfectionefficiencywasdeterminedbyqRT-PCRandWesternBlotanalysis.
TransfectionProtocolandMSDS:
DownloadAltogenBiosystemsAltoFect™TransfectionProtocol:[PDF]
DownloadMSDS:[PDF]
Data:
Figure1. CyclophilinBsilencingefficiencywasdeterminedbyqRT-PCRinprimaryneuronalculturetransfectedbyCyclophilinBsiRNAornon-silencingsiRNAcontrolfollowingtherecommendedAltoFecttransfectionprotocol.CyclophilinmRNAexpressionlevelsweremeasured48hourspost-transfection.18SrRNAlevelswereusedtonormalizetheCyclophilinBdata.Valuesarenormalizedtountreatedsample.Dataarepresentedasmeans±SD(n=6).
Figure2. ProteinexpressionofCyclophilinBinprimaryneuronalcells.DNAplasmidexpressingCyclophilinBorsiRNAtargetingCyclophilinBweretransfectedintoprimaryneuronalcellsfollowingAltoFect™transfectionprotocol.At72hourspost-transfectionthecellswereanalyzedbyWesternBlotforproteinexpressionlevels(normalizedbytotalprotein,10µgoftotalproteinloadedpereachwell).Untreatedcellsusedasanegativecontrol.
AboutAltogenBiosystems:
AltogenBiosystemsisalifesciencescompany thatoffers celltype-specificandpre-optimizedtransfection products, electroporationkits,and invivo deliveryreagents.Advancedformulationofreagentsandoptimizedtransfectionprotocolsprovideefficientintracellulardeliveryofprotein,DNA,mRNA,shRNA andsiRNAmolecules.Readmore abouttransfectiontechnologyatAltogen’s TransfectionResource.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpreclinicalresearch,INDapplications,anddrugdevelopment.BIOLOGyCROservices include:Over60xenograftmodels,developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 0.05ml(Catalog#3000)
- 0.5ml(Catalog#3001)
- 1.5ml(Catalog#3002)
- 8.0ml(Catalog#3003)