Description
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Non-silencingcontrolsiRNA(5nmol)
- Non-silencingtransfectioncontrolsiRNA
- Kitincludes5nmolofready-to-usechemicallysynthesizedsiRNA
- ScrambledsequencesiRNA
- Nottargetinganyknownhuman/mousegenes
- TransfectioncontrolforchemicaltransfectionandsiRNAelectroporationapplications
- DevelopedandmanufacturedbyAltogenBiosystems
siRNATransfection:
GenesilencingbyRNAInterference(RNAi)isapowerfulresearchtoolforstudyinggenefunctioninmammaliancells.RNAiisaBIOLOGicalphenomenonbywhichdoublestrandedRNA(dsRNA)specificallyreducesgeneexpressionofitscorrespondinggene.PotentinhibitionofspecificgeneexpressionisexperimentallyachievedbythetransfectionofsmallinterferingRNA(siRNA),whichbindsRISCcomplexandcausedegradationoftargetcomplementarymRNAmoleculesinthecell.Therefore,successful,potentRNAiexperimentationisdependentuponthehighlyefficientdeliveryofthesiRNAintocellsbytransfectionofstableandfunctionalsiRNAmolecules.MostDNAtransfectionreagentsareincompatIBLewithsiRNA,sensitivetoserum,orarecapableofinducingcytotoxicity,whichnegativelyaffectsgeneexpressionstudies.TransfectionReagentshavebeendevelopedandoptimizedspecificallyforusewithsiRNAtransfectionandallowtransfectioninthepresenceofserumwithoutloweringtransfectionefficiencyorreducingcellviABIlity.Ready-to-runtransfectionprotocolseliminatetheneedforextensivesiRNAoptimizationexperiments.AltogenBiosystemskitsarealsocompatiblefortransfectionofmicroRNA(miRNA)molecules.
Selected siRNAcitationreferences:
- CellDeathDiffer.2016.23(5):787-800.UnexpectedrequirementforabindingpartnerofthesyntaxinfamilyinphagocytosisbymurinetesticularSertolicells.Dongetal[PDF]
- BritishJournalofCancer.2012 107(3):516-26.TIGARinducesp53-mediatedcell-cycle…Madanetal[PDF]
- JounalofBiologicalChemistry.2012287(4):2907.Chaperoningofmutantp53protein…Gognaetal [PDF]
Non-silencingcontrolsiRNA
AltogenBiosystems:
AltogenBiosystemsisalifesciencescompanydedicatedtothedevelopment,marketingandmanufactureofcelltypespecifictransfectionreagents.EfficientdeliveryofDNA,RNA,siRNA,andmicroRNAenabledbyadvancedformulationofreagentsandpeculiardesignofprotocols.AltogenBiosystemsoffersacompletetransfectionsystemforabroadrangeofcelllines.Allreagentsarefunctionallytestedtobehighlyreproducible,serumcompatible,inducelowtoxicity,andcanbeusedforco-transfectionexperiments,andhighthroughputapplications.
AltogenLabsResearchServices:
AltogenLabsprovidesGLP-compliantcontractresearchstudiesforpre-clinicalresearch,INDapplications,anddrugdevelopment.BiologyCROservices include:Xenograftmodels(30+),developmentofstablecelllines,ELISAassaydevelopment,cell-basedandtissuetargetedRNAistudies,safetypharm/toxassays,andotherstudies(visitAltogenLabs.com).
VolumeOptions:
- 5nmol(Catalog#4063)